Rapid and Accurate Detection of Plant miRNAs by Liquid Northern Hybridization

International Journal of Molecular Sciences

Xiaosu Wang¹  Yongao Tong¹ 

[1] Lab of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China;

关键词: miRNA;    liquid Northern hybridization;    Oryza sativa;    small RNA;    oligonucleotide probes;   

DOI  :  10.3390/ijms11093138

来源: mdpi

【 摘 要 】

Northern blot analysis is a powerful research tool for discovery, validation and expression of genes, and is currently widely used to detect microRNA (miRNA) accumulation. However, the traditional Northern blot procedure, which is based on a support membrane, is overly elaborate and time-consuming, although it is unsurpassed in accuracy for determining the sizes and amounts of multiple small RNAs sharing high sequence identity. Here we present an alternative method derived from plant miRNAs, liquid Northern hybridization, using fluorescently labeled oligonucleotide probes and characterized by simple and specific miRNA determination and quantitation. The entire detection process is completed within a few hours, and multiple miRNAs can be simultaneously detected in a single experiment.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.