Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment

Sensors

Efstratios Komaitis²  Efstathios Vasiliou²  Gerasimos Kremmydas¹  Dimitrios G. Georgakopoulos¹ 

[1] Microbiology Laboratory, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece; E-Mails:;Chemistry Laboratory, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece; E-Mails:

关键词: Vibrio fischeri;    toxicity;    flow injection;   

DOI  :  10.3390/s100807089

来源: mdpi

【 摘 要 】

This paper describes the development of an automated Flow Injection analyzer for water toxicity assessment. The analyzer is validated by assessing the toxicity of heavy metal (Pb²⁺, Hg²⁺ and Cu²⁺) solutions. One hundred μL of a Vibrio fischeri suspension are injected in a carrier solution containing different heavy metal concentrations. Biosensor cells are mixed with the toxic carrier solution in the mixing coil on the way to the detector. Response registered is % inhibition of biosensor bioluminescence due to heavy metal toxicity in comparison to that resulting by injecting the Vibrio fischeri suspension in deionised water. Carrier solutions of mercury showed higher toxicity than the other heavy metals, whereas all metals show concentration related levels of toxicity. The biosensor’s response to carrier solutions of different pHs was tested. Vibrio fischeri’s bioluminescence is promoted in the pH 5–10 range. Experiments indicate that the whole cell biosensor, as applied in the automated fluidic system, responds to various toxic solutions.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.