Integral Kinetic Model for Studying Quercetin Degradation and Oxidation as Affected by Cholesterol During Heating

International Journal of Molecular Sciences

John-Tung Chien¹  Da-Jung Hsu¹  Baskaran Stephen Inbaraj¹ 

[1]Department of Food Science, Fu Jen University, Taipei 242, Taiwan

关键词: quercetin;    cholesterol oxidation;    kinetic study;   

DOI  :  10.3390/ijms11082805

来源: mdpi

【 摘 要 】

The degradation and oxidation of quercetin, as affected by cholesterol during heating at 150 °C, was kinetically studied using non-linear regression models. Both TLC and HPLC were used to monitor the changes of quercetin, cholesterol and cholesterol oxidation products (COPs) during heating. The formation of COPs, including triol, 7-keto, 7α-OH and 7β-OH, was completely inhibited during the initial 30 minute heating period in the presence of 0.02% quercetin, accompanied by reduction in cholesterol peroxidation and degradation. However, the quercetin degradation or oxidation proceeded fast, with the rate constants (h⁻¹) in the presence of nitrogen, oxygen and the combination of oxygen and cholesterol being 0.253, 0.868 and 7.17, respectively. When cholesterol and quercetin were heated together, the rate constants (h⁻¹) of cholesterol peroxidation, epoxidation and degradation were 1.8 × 10⁻⁴, 0.016 and 0.19, respectively. The correlation coefficients (r²) for all the oxidative and degradation reactions ranged from 0.82–0.99. The kinetic models developed in this study may be used to predict the degradation and oxidation of quercetin as affected by cholesterol during heating.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.