Characterization of the Exopolysaccharide Produced by Salipiger mucosus A3T, a Halophilic Species Belonging to the Alphaproteobacteria, Isolated on the Spanish Mediterranean Seaboard

Marine Drugs

Inmaculada Llamas²  Juan Antonio Mata²  Richard Tallon³  Philippe Bressollier³  Mar໚ C. Urdaci¹  Emilia Quesada² 

[1] Laboratorie de Microbiologie et Biochimie Appliquée, ENITA de Bordeaux, 1 Cours du Général de Gaulle, BP 201, 33175 Gradignan, France; E-Mail:;Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Cartuja Campus, University of Granada, 18071 Granada, Spain; E-Mails:;IUT, Département Génie Biologique, allée André Maurois, 87065 Limoges, France; E-Mails:

关键词: exopolysaccharides;    Salipiger mucosus;    halophilic bacteria;    fucose;    sulfates;   

DOI  :  10.3390/md8082240

来源: mdpi

【 摘 要 】

We have studied the exopolysaccharide produced by the type strain of Salipiger mucosus, a species of halophilic, EPS-producing (exopolysaccharide-producing) bacterium belonging to the Alphaproteobacteria. The strain, isolated on the Mediterranean seaboard, produced a polysaccharide, mainly during its exponential growth phase but also to a lesser extent during the stationary phase. Culture parameters influenced bacterial growth and EPS production. Yield was always directly related to the quantity of biomass in the culture. The polymer is a heteropolysaccharide with a molecular mass of 250 kDa and its components are glucose (19.7%, w/w), mannose (34%, w/w), galactose (32.9%, w/w) and fucose (13.4%, w/w). Fucose and fucose-rich oligosaccharides have applications in the fields of medicine and cosmetics. The chemical or enzymatic hydrolysis of fucose-rich polysaccharides offers a new efficient way to process fucose. The exopolysaccharide in question produces a solution of very low viscosity that shows pseudoplastic behavior and emulsifying activity on several hydrophobic substrates. It also has a high capacity for binding cations and incorporating considerable quantities of sulfates, this latter feature being very unusual in bacterial polysaccharides.

【 授权许可】

CC BY   
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