In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts

Molecules

Virgínia Sbrugnera Nazato¹  Leandro Rubem-Mauro¹  Nathalia Aparecida Gatto Vieira¹  Dimas Dos Santos Rocha¹  Magali Glauzer Silva¹  Patricia Santos Lopes¹  Cháriston André Dal-Belo¹  Jose Carlos Cogo¹  Marcio Galdino Dos Santos¹  Maria Alice Da Cruz-Höfling¹ 

[1] ¹Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil

关键词: Bothrops jararacussu;    Crotalus durissus terrificus;    medicinal plant extract;    myotoxicity;    neurotoxicity;   

DOI  :  10.3390/molecules15095956

来源: mdpi

【 摘 要 】

Extracts from Dipteryx alata bark obtained with different solvents (hexane, dichloromethane, ethyl acetate and methanol) were mixed in vitro with Bothrops jararacussu (Bjssu, 40 μg/mL) and Crotalus durissus terrificus (Cdt, 15 μg/mL) snake venoms, and applied to a mouse phrenic nerve-diaphragm preparation to evaluate the possible neutralization of venom effects. Cdt venom neurotoxic effect was not inhibited by any of the extracts, while the neurotoxic and myotoxic actions of Bjssu venom were decreased by the methanolic extract. This inhibition appears to be augmented by tannins. Dichloromethane bark extract inhibited ~40% of Bjssu venom effects and delayed blockade induced by Cdt. The methodology used to determine which extract was active allows inferring that: (i) phenolic acids and flavonoids contained in the methanolic extract plus tannins were responsible mostly for neutralization of Bjssu effects; (ii) terpenoids from the dichloromethane extract may participate in the anti-Cdt and anti-Bjssu venom effects; (iii) a given extract could not inhibit venoms from different species even if those belong to the same family, so it is improper to generalize a certain plant as antiophidian; (iv) different polarity extracts do not present the same inhibitory capability, thus demonstrating the need for characterizing both venom pharmacology and the phytochemistry of medicinal plant compounds

【 授权许可】

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This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.