FLP/FRT Recombination from Yeast: Application of a Two Gene Cassette Scheme as an Inducible System in Plants

Sensors

Murali R. Rao²  Hong S. Moon²  Tobias M. H. Schenk¹  Dirk Becker¹  Mitra Mazarei² 

[1] Biocentre Klein Flottbek, Developmental Biology and Biotechnology, University of Hamburg, Hamburg, Germany; E-Mails:;Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA; E-Mails:

关键词: FLP/FRT;    site-specific recombination;    heat shock;    GUS reporter;    tobacco;    Arabidopsis;    phytosensing;   

DOI  :  10.3390/s100908526

来源: mdpi

【 摘 要 】

Phytosensors are plants that are genetically engineered for sensing and reporting the presence of a specific contaminant, including agriculturally important biological agents. Phytosensors are constructed by transforming plants to contain specific biotic- or abiotic-inducible promoters fused to a reporter gene. When such transgenic plants encounter the target biotic or abiotic agent, the specific inducible promoter is triggered and subsequently drives the expression of the reporter gene, which produces a signal for detection. However, several systems lack robustness, rapid induction and promoter strength. Here, we tested the FLP/FRT recombination system in a construct containing a two gene cassette organization and examined its potential in transgenic Arabidopsis and tobacco plants using a β-glucuronidase (GUS) reporter. In this model system, a heat-shock inducible promoter was employed to control the expression of the FLP recombinase gene. Upon heat induction and subsequent active FLP-mediated excision event, the GUS gene was placed in close proximity to the 35S promoter resulting in an active GUS reporter expression. Our results demonstrate that the two gene cassette scheme of inducible FLP/FRT recombination system is functional in tobacco and Arabidopsis, providing additional insights into its possible application in phytosensing such as creating strong readout capabilities.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.