The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet

International Journal of Molecular Sciences

Todd W. Mitchell²  Nigel Turner¹  Paul L. Else²  Anthony J. Hulbert³  John A. Hawley⁶  Jong Sam Lee⁴  Clinton R. Bruce⁵ 

[1] Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; E-Mail:;School of Health Sciences, University of Wollongong, NSW 2522, Australia; E-Mail:;School of Biological Sciences, University of Wollongong, NSW 2522, Australia; E-Mail:;Department of Physical Education, Daegu University, Daegu, South Korea; E-Mail:;Baker IDI Heart & Diabetes Institute, Melbourne, Victoria 3004 Australia; E-Mail:;School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia; E-Mail:

关键词: electrospray-ionization mass spectrometry;    endurance;    exercise training;    fatty acids;    lipidomics;   

DOI  :  10.3390/ijms11103954

来源: mdpi

【 摘 要 】

The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.