Toxic Effects of Domoic Acid in the Seabream Sparus aurata

Marine Drugs

Isabel Nogueira²  Alexandre Lobo-da-Cunha²  António Afonso²  Socorro Rivera¹  Joana Azevedo²  Rogério Monteiro²  Rosa Cervantes¹  Ana Gago-Martinez¹ 

[1] Departamento de Química Analítica, Facultad de Química, As Lagoas Marcosende C.P. 36210, Spain; E-Mails:;CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Rua dos Bragas 177-289, 4150-123 Porto, Portugal; E-Mails:

关键词: domoic acid;    amnesic shellfish poisoning;    Pseudo-nitzschia;    algal neurotoxin;    Sparus aurata;   

DOI  :  10.3390/md8102721

来源: mdpi

【 摘 要 】

Neurotoxicity induced in fish by domoic acid (DA) was assessed with respect to occurrence of neurotoxic signs, lethality, and histopathology by light microscopy. Sparus aurata were exposed to a single dose of DA by intraperitoneal (i.p.) injection of 0, 0.45, 0.9, and 9.0 mg DA kg⁻¹ bw. Mortality (66.67 ± 16.67%) was only observed in dose of 9.0 mg kg⁻¹ bw. Signs of neurological toxicity were detected for the doses of 0.9 and 9.0 mg DA kg⁻¹ bw. Furthermore, the mean concentrations (±SD) of DA detected by HPLC-UV in extracts of brain after exposure to 9.0 mg DA kg⁻¹ bw were 0.61 ± 0.01, 0.96 ± 0.00, and 0.36 ± 0.01 mg DA kg⁻¹ tissue at 1, 2, and 4 hours. The lack of major permanent brain damage in S. aurata, and reversibility of neurotoxic signs, suggest that lower susceptibility to DA or neuronal recovery occurs in affected individuals.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland