期刊论文详细信息
Marine Drugs
TFA and EPA Productivities of Nannochloropsis salina Influenced by Temperature and Nitrate Stimuli in Turbidostatic Controlled Experiments
Maren Hoffmann1  Kai Marxen1  R࿍iger Schulz2 
[1]Forschungs- und Technologiezentrum Westküste der Universität Kiel, Hafentörn 1, 25761 Büsum, Germany
[2] E-Mails:
[3]Botanisches Institut der Universität Kiel, Am Botanischen Garten 1–9, 24098 Kiel, Germany
[4] E-Mail:
关键词: microalgae;    ratio unsaturated/saturated FA;    growth rate;    total fatty acids;    eicosapentaenoic acid;   
DOI  :  10.3390/md8092526
来源: mdpi
PDF
【 摘 要 】

The influence of different nitrate concentrations in combination with three cultivation temperatures on the total fatty acids (TFA) and eicosapentaenoic acid (EPA) content of Nannochloropsis salina was investigated. This was done by virtue of turbidostatic controlled cultures. This control mode enables the cultivation of microalgae under defined conditions and, therefore, the influence of single parameters on the fatty acid synthesis of Nannochloropsis salina can be investigated. Generally, growth rates decreased under low nitrate concentrations. This effect was reinforced when cells were exposed to lower temperatures (from 26 °C down to 17 °C). Considering the cellular TFA concentration, nitrate provoked an increase of TFA under nitrate limitation up to 70% of the biological dry mass (BDM). In contrast to this finding, the EPA content decreased under low nitrate concentrations. Nevertheless, both TFA and EPA contents increased under a low culture temperature (17 °C) compared to moderate temperatures of 21 °C and 26 °C. In terms of biotechnological production, the growth rate has to be taken into account. Therefore, for both TFA and EPA production, a temperature of 17 °C and a nitrate concentration of 1800 μmol L−1 afforded the highest productivities. Temperatures of 21 °C and 26 °C in combination with 1800 μmol L−1 nitrate showed slightly lower TFA and EPA productivities.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

【 预 览 】
附件列表
Files Size Format View
RO202003190052184ZK.pdf 204KB PDF download
  文献评价指标  
  下载次数:4次 浏览次数:5次