Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2

International Journal of Molecular Sciences

Pornpimon Kanjanavas³  Sintawee Khuchareontaworn³  Paisarn Khawsak³  Arda Pakpitcharoen³  Khajeenart Pothivejkul²  Somchai Santiwatanakul¹  Kenji Matsui⁴  Tadahiko Kajiwara⁴ 

[1] Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand; E-Mail:;Department of Biology, Faculty of Sciences, Srinakharinwirot University, Bangkok 10110, Thailand; E-Mail:;Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand; E-Mails:;Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; E-Mail:

关键词: lipase;    organic tolerant;    detergent tolerant;    Bacillus;    thermotolerant;   

DOI  :  10.3390/ijms11103783

来源: mdpi

【 摘 要 】

The aim of this study was to characterize the organic solvent and detergent tolerant properties of recombinant lipase isolated from thermotolerant Bacillus sp. RN2 (Lip-SBRN2). The isolation of the lipase-coding gene was achieved by the use of inverse and direct PCR. The complete DNA sequencing of the gene revealed that the lip-SBRN2 gene contains 576 nucleotides which corresponded to 192 deduced amino acids. The purified enzyme was homogeneous with the estimated molecular mass of 19 kDa as determined by SDS-PAGE and gel filtration. The Lip-SBRN2 was stable in a pH range of 9–11 and temperature range of 45–60 °C. The enzyme was a non metallo-monomeric protein and was active against pNP-caprylate (C8) and pNP-laurate (C12) and coconut oil. The Lip-SBRN2 exhibited a high level of activity in the presence of 108% benzene, 102.4% diethylether and 112% SDS. It is anticipated that the organic solvent and detergent tolerant enzyme secreted by Bacillus sp. RN2 will be applicable as catalysts for reaction in the presence of organic solvents and detergents.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.