Antimicrobial Metabolites from the Endophytic Fungus Pichia guilliermondii Isolated from Paris polyphylla var. yunnanensis

Molecules

Jianglin Zhao¹  Yan Mou¹  Tijiang Shan¹  Yan Li¹  Ligang Zhou¹  Mingan Wang¹ 

[1] ¹College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China ²College of Science, China Agricultural University, Beijing 100193, China ³College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China

关键词: endophytic fungus;    Pichia guilliermondii;    Paris polyphylla var. yunnanensis;    steroids;    helvolic acid;    antimicrobial activity;   

DOI  :  10.3390/molecules15117961

来源: mdpi

【 摘 要 】

Three steroids and one nordammarane triterpenoid were isolated for the first time from the endophytic fungus Pichia guilliermondii Ppf9 derived from the medicinal plant Paris polyphylla var. yunnanensis. By means of physicochemical and spectrometric analysis, they were identified as ergosta-5,7,22-trienol (1), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), ergosta-7,22-dien-3β,5α,6β-triol (3), and helvolic acid (4). Both micro-dilution-colorimetric and spore germination assays were employed to evaluate their antimicrobial activity. Among them, helvolic acid (4) exhibited the strongest antibacterial activity against all test bacteria, with MIC values ranging from 1.56 µg/mL to 50 µg/mL, and IC₅₀ values from 0.98 µg/mL to 33.19 µg/mL. It also showed strong inhibitory activity on the spore germination of Magnaporthe oryzae with an IC₅₀ value of 7.20 µg/mL. Among the three steroids, 5α,8α-epidioxyergosta-6,22-dien-3β-ol (2) exhibited relatively strong antimicrobial activity. The results suggest that the endophytic fungus Pichia guillermondii Ppf9 could be a candidate for producing helvolic acid, and the metabolites from this fungus could be potentially developed as antimicrobial agents in the future.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.