A Combined Experimental and Theoretical Study on the Immunoassay of Human Immunoglobulin Using a Quartz Crystal Microbalance

Sensors

Po-Jen Liao²  Jeng-Shian Chang¹  Sheng D. Chao¹  Hung-Chi Chang²  Kuan-Rong Huang²  Kuang-Chong Wu² 

[1] Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan;

关键词: biosensor;    Quartz Crystal Microbalance;    Finite Element Method (FEM);    basic kinetic analysis;    human IgG1;   

DOI  :  10.3390/s101211498

来源: mdpi

【 摘 要 】

We investigate a immunoassay biosensor that employs a Quartz Crystal Microbalance (QCM) to detect the specific binding reaction of the (Human IgG1)-(Anti-Human IgG1) protein pair under physiological conditions. In addition to experiments, a three dimensional time domain finite element method (FEM) was used to perform simulations for the biomolecular binding reaction in microfluidic channels. In particular, we discuss the unsteady convective diffusion in the transportation tube, which conveys the buffer solution containing the analyte molecules into the micro-channel where the QCM sensor lies. It is found that the distribution of the analyte concentration in the tube is strongly affected by the flow field, yielding large discrepancies between the simulations and experimental results. Our analysis shows that the conventional assumption of the analyte concentration in the inlet of the micro-channel being uniform and constant in time is inadequate. In addition, we also show that the commonly used procedure in kinetic analysis for estimating binding rate constants from the experimental data would underestimate these rate constants due to neglected diffusion processes from the inlet to the reaction surface. A calibration procedure is proposed to supplement the basic kinetic analysis, thus yielding better consistency with experiments.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.