Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry

Materials

Martin Huth¹  Samira Hertrich¹  Gabor Mezo²  Emilia Madarasz³ 

[1]Ludwig-Maximilians-Universität, Department für Physik and CeNS, Geschwister-Scholl-Platz 1, 80539 Munich, Germany

[2] E-Mails:

[3]Research Group of Peptide Chemistry, Hungarian Academy of Science, Eötvös L. University, Pazmany P. stny. 1/A, 1117 Budapest, Hungary

[4] E-Mail:

[5]Laboratory of Cellular and Developmental Neurobiology, Institute of Experimental Medicine of Hungarian Academy of Science, Szigony u. 43, Budapest, H-1083, Hungary

[6] E-Mail:

关键词: supported lipid bilayers;    biomimetics;    bionanotechnology;    functional coatings;    self-assembly;   

DOI  :  10.3390/ma3114994

来源: mdpi

【 摘 要 】

We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cyclo[RGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence. Biotinylated AK-cyclo[RGDfC] is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration and packing of the coating is quantified by X-ray and neutron reflectometry experiments. AK-cyclo[RGDfC] binds to the streptavidin interlayer in a stretched-out on edge configuration. The highly packed configuration with only 12% water content maximizes the number of accessible adhesion sites. Enhanced cell spreading of neural stem cells was observed for AK-cyclo[RGDfC] functionalized bilayers. Due to the large variety of surfaces which can be coated by physisorption of lipid bilayers, this approach is of general interest for the fabrication of biocompatible surfaces.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.