Tomato Polyphenol Oxidase B Is Spatially and Temporally Regulated during Development and in Response to Ethylene

Molecules

Sally M. Newman¹  Piyada Tantasawat¹ 

[1] ¹Department of Plant Breeding and Genetics, 252 Emerson Hall, Cornell University, Ithaca, NY 14853, USA ²Suranaree University of Technology, 111 University Ave., Muang District, Nakhon Ratchasima 30000, Thailand ^†Present address: Syngenta Biotechnology, 3054 Cornwallis Rd., Research Triangle Park, NC 27709, USA

关键词: Differential expression;    ethylene;    GUS;    PPO;    promoter analysis;    Solanum lycopersicum;    transgenic;   

DOI  :  10.3390/molecules16010493

来源: mdpi

【 摘 要 】

Plant polyphenol oxidases (PPOs) are ubiquitous plastid-localized enzymes. A precise analysis of PPO function in plants has been complicated by the presence of several family members with immunological cross reactivity. Previously we reported the isolation of genomic clones coding for the seven members of the tomato (Solanum lycopersicum) PPO family (A, A’, B, C, D, E, and F). Here we report the complex spatial and temporal expression of one of the members, PPO B. The PPO B promoter was sequenced and subjected to homology analysis. Sequence similarities were found to nucleotide sequences of genes encoding enzymes/proteins active in the following systems: phenylpropanoid biosynthesis, signal transduction and responsiveness to hormones and stresses, fruit and seed proteins/enzymes, and photosynthesis. Chimeric gene fusions were constructed linking PPO B 5' flanking regions to the reporter gene, b-glucuronidase (GUS). The resultant transgenic plants were histochemically analyzed for GUS activity in various vegetative and reproductive tissues, and evaluated for PPO B responsiveness to ethylene induction. It was shown that PPO B expression was tissue specific, developmentally regulated, ethylene induced, and localized predominantly to mitotic or apoptotic tissues.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.