IL8 and Cathepsin B as Melanoma Serum Biomarkers

International Journal of Molecular Sciences

Hongtao Zhang¹  Ting Fu¹  Suzanne McGettigan³  Suresh Kumar¹  Shujing Liu¹  David Speicher²  Lynn Schuchter³ 

[1] Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; E-Mails:;The Wistar Institute, 3601 Spruce St., Philadelphia, PA 19104, USA; E-Mail:;Division of Hematology-Oncology, HUP, 16 Penn Tower, Philadelphia, PA 19104, USA; E-Mails:

关键词: IL-8;    cathepsin B;    melanoma;    serum interference;   

DOI  :  10.3390/ijms12031505

来源: mdpi

【 摘 要 】

Melanoma accounts for only a small portion of skin cancer but it is associated with high mortality. Melanoma serum biomarkers that may aid early diagnosis or guide therapy are needed clinically. However, studies of serum biomarkers have often been hampered by the serum interference that causes false readouts in immunological tests. Here we show that, after using a special buffer to eliminate the serum interference, IL-8 and cathepsin B levels were significantly elevated in melanoma patients (p < 0.05). More importantly, the combination of IL-8 and cathepsin B were also studied as a prognosis marker for melanoma mortality. Our study provides a novel approach to examine serum biomarkers.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.