PLGA-Based Microparticles for the Sustained Release of BMP-2

Polymers

Giles T. S. Kirby²  Lisa J. White²  Cheryl V. Rahman²  Helen C. Cox²  Omar Qutachi²  Felicity R. A. J. Rose²  Dietmar W. Hutmacher¹  Kevin M. Shakesheff² 

[1] Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia; E-Mail:;School of Pharmacy, University of Nottingham, University Park, Nottingham, UK; E-Mails:

关键词: poly(lactic-co-glycolic acid);    PLGA;    poly(ethylene glycol) PEG;    MC3T3;    differentiation;    BMP-2;    microparticles;    microspheres;    controlled release;   

DOI  :  10.3390/polym3010571

来源: mdpi

【 摘 要 】

The development of growth factor delivery strategies to circumvent the burst release phenomenon prevalent in most current systems has driven research towards encapsulating molecules in resorbable polymer matrices. For these polymer release techniques to be efficacious in a clinical setting, several key points need to be addressed. This present study has investigated the encapsulation of the growth factor, BMP-2 within PLGA/PLGA-PEG-PLGA microparticles. Morphology, size distribution, encapsulation efficiency and release kinetics were investigated and we have demonstrated a sustained release of bioactive BMP-2. Furthermore, biocompatibility of the PLGA microparticles was established and released BMP-2 was shown to promote the differentiation of MC3T3-E1 cells towards the osteogenic lineage to a greater extent than osteogenic supplements (as early as day 10 in culture), as determined using alkaline phosphatase and alizarin red assays. This study showcases a potential BMP-2 delivery system which may now be translated into more complex delivery systems, such as 3D, mechanically robust scaffolds for bone tissue regeneration applications.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.