International Journal of Molecular Sciences | |
Evaluation of Potential Reference Genes for Relative Quantification by RT-qPCR in Different Porcine Tissues Derived from Feeding Studies | |
Qimeng Li3  Konrad Johann Domig2  Thomas Ettle3  Wilhelm Windisch1  Christiane Mair3  | |
[1] Chair of Animal Nutrition, Technische Universitaet Muenchen-Weihenstephan, Liesel-Beckmann-Strasse 6, 85350 Freising-Weihenstephan, Germany; E-Mail:;Department of Food Sciences and Technology, Institute for Food Science, University of Natural Resources and Life Sciences, 1190 Vienna, Austria; E-Mail:;Institute of Animal Nutrition, Products, and Nutrition Physiology (APN), University of Natural Resources and Life Sciences, 1190 Vienna, Austria; E-Mails: | |
关键词: sus scrofa; RT-qPCR; reference genes; gene stability; | |
DOI : 10.3390/ijms12031727 | |
来源: mdpi | |
【 摘 要 】
Five potential reference genes for RT-qPCR application, namely histone H3, beta-actin, GAPDH, ubiquitin and 18S rRNA, were evaluated for normalization of gene expression in four selected tissues (liver, kidney, thyroid and abdominal fat). Tissues were derived from fattening pigs exposed to different amounts and type of dietary iodine. Two software applications (
【 授权许可】
CC BY
© 2011 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190050376ZK.pdf | 138KB | download |