An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System

Sensors

Gustavo A. Alonso²  Rocio B. Dominguez²  Jean-Louis Marty¹ 

[1] Université de Perpignan Via Domitia, IMAGES EA4218, Centre de Phytopharmacie, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France; E-Mail:;Centro de Investigación y de Estudios Avanzados del Intituto Politecnico Nacional, Av. Instituto Politécnico Nacional 2508, Mexico; E-Mails:

关键词: biosensor;    enzyme inhibition;    flow system;    multi-commuted;    organo-phosphorus insecticides;    potentiostat;   

DOI  :  10.3390/s110403791

来源: mdpi

【 摘 要 】

An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Multi-commuted Flow Analysis system (MCFA) was selected and developed to carry out assays with an improved inhibition method to detect the insecticides chlorpyrifos oxon (CPO), chlorfenvinfos (CFV) and azinphos methyl-oxon (AZMO). The system manifold comprised a peristaltic pump, a set of seven electronic valves controlled by a personal computer electronic interface and software based on LabView® to control the sample dilutions into the cell. The inhibition method consists in the injection of the insecticide when the enzyme activity has reached the plateau of the current; with this method the incubation time is avoided. A potentiostat was developed to measure the response from the enzymatic biosensor. Low limits of detection of 10 nM for CPO, CFV, and AZMO were achieved.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.