Effects of High Salt Stress on Secondary Metabolite Production in the Marine-Derived Fungus Spicaria elegans

Marine Drugs

Yi Wang¹  Zhenyu Lu¹  Kunlai Sun¹ 

[1] Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China;

关键词: Spicaria elegans;    high salt stress;    secondary metabolites;   

DOI  :  10.3390/md9040535

来源: mdpi

【 摘 要 】

To obtain structurally novel and bioactive natural compounds from marine-derived microorganisms, the effect of high salt stress on secondary metabolite production in the marine-derived fungal strain, Spicaria elegans KLA-03, was investigated. The organism, which was isolated from marine sediment, produced different secondary metabolites when cultured in 3% and 10% saline conditions. Four characteristic metabolites, only produced in the 10% salinity culture, were isolated, and their structures were identified as (2E,2′Z)-3,3′-(6,6′-dihydroxybiphenyl-3,3′-diyl)diacrylic acid (1), aspulvinone E (2), aspochalasin E (3) and trichodermamide B (6), according to their 1D and 2D NMR spectra. Compound 1 is a new compound. High salt stress may therefore be a promising means to induce the production of new and chlorinated compounds in halotolerant fungi. Compound 1 showed moderate antibacterial activity against Pseudomonas aeruginosa and Escherichia coli with minimum inhibitory concentration (MIC) values of 0.038 and 0.767 mM, respectively.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.