Pharmaceutics | |
Live Cell |
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Beverley Isherwood2  Paul Timpson1  Ewan J McGhee1  Kurt I Anderson1  Marta Canel3  Alan Serrels3  Valerie G Brunton3  | |
[1]Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK | |
[2] E-Mails: | |
[3]Advanced Science & Technology Laboratory, AstraZeneca R&D Charnwood, Loughborough LE11 5RH, UK | |
[4] E-Mail: | |
[5]Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, UK | |
[6] E-Mails: | |
关键词: imaging; intravital; high-content; temporal; fluorescence; drug discovery; biomarkers; translation; efficacy; | |
DOI : 10.3390/pharmaceutics3020141 | |
来源: mdpi | |
【 摘 要 】
Dynamic regulation of specific molecular processes and cellular phenotypes in live cell systems reveal unique insights into cell fate and drug pharmacology that are not gained from traditional fixed endpoint assays. Recent advances in microscopic imaging platform technology combined with the development of novel optical biosensors and sophisticated image analysis solutions have increased the scope of live cell imaging applications in drug discovery. We highlight recent literature examples where live cell imaging has uncovered novel insight into biological mechanism or drug mode-of-action. We survey distinct types of optical biosensors and associated analytical methods for monitoring molecular dynamics,
【 授权许可】
CC BY
© 2011 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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RO202003190050237ZK.pdf | 2093KB | download |