Cell Migration and Invasion Assays as Tools for Drug Discovery

Pharmaceutics

Keren I. Hulkower¹ 

关键词: cell migration;    cell invasion;    high throughput screening;    high content analysis;    phenotypic assays;    drug discovery;    3-dimensional assays;    wound healing;    cancer metastasis;   

DOI  :  10.3390/pharmaceutics3010107

来源: mdpi

【 摘 要 】

Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.