Molecular Characterization of Arabinoxylans from Hull-Less Barley Milling Fractions

Molecules

Xueling Zheng¹  Limin Li¹ 

[1] Grain College, Henan University of Technology, Zhengzhou 450052, Henan Province, China

关键词: arabinoxylan;    hull-less barley;    size exclusion chromatography analysis;    dynamic light scattering analysis;   

DOI  :  10.3390/molecules16042743

来源: mdpi

【 摘 要 】

Arabinoxylans were prepared from different hull-less barley milling fractions (bran, shorts and flour). The yields of hull-less bran arabinoxylan (HBB-AX), shorts arabinoxylan (HBS-AX) and flour arabinoxylan (HBF-AX) were 8.42%, 4.08% and 2.13% respectively. Sugar composition analysis showed that arabinose and xylose were the main sugars. HBF-AX had the highest Ara/Xyl ratio, followed by HBS-AX and HBB-AX. Size exclusion chromatography analysis (HPSEC) showed that HBF-AX had the highest molecular weight, followed by HBS-AX and HBB-AX, which had the lowest molecular weight. Aqueous solutions of HBB-AX, HBS-AX and HBF-AX had higher molecular weight(Mw) than in 0.5 mol/L NaOH and 1.0 mol/L NaOH. Dynamic light scattering analysis (DLS) showed that HBB-AX, HBS-AX and HBF-AX had existed in two states in distilled water, 0.5 mol/LNaOH, 1.0 mol/L NaOH, and DMSO/H₂O (90/10), an unaggregated state and an aggregated state, with the latter predominating.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.