Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco Rhizosphere

International Journal of Molecular Sciences

Fengli Jin¹  Yanqin Ding¹  Wei Ding³  M.S. Reddy²  W.G. Dilantha Fernando⁴ 

[1] Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China; E-Mails:;Department of Entomology and Plant Pathology, 209 Life Sciences Bldg, Auburn University, Auburn, AL 36849, USA; E-Mail:;Zunyi Tobacco Company, Guizhou 564700, China; E-Mail:;Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; E-Mail:

关键词: genetic diversity;    antagonistic bacteria;    Phytophthora nicotianae;    tobacco;    rhizosphere;   

DOI  :  10.3390/ijms12053055

来源: mdpi

【 摘 要 】

The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.