Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

Marine Drugs

Leandro Silva Costa¹  Cinthia Beatrice Silva Telles¹  Ruth Medeiros Oliveira¹  Leonardo Thiago Duarte Barreto Nobre¹  Nednaldo Dantas-Santos¹  Rafael Barros Gomes Camara¹  Mariana Santana Santos Pereira Costa¹  Jailma Almeida-Lima¹  Raniere Fagundes Melo-Silveira¹  Ivan Rui Lopes Albuquerque¹  Edda Lisboa Leite¹ 

[1] Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil; E-Mails:

关键词: fucoidan;    sulfated polysaccharides;    anticancer;    apoptosis-inducing factor (AIF);   

DOI  :  10.3390/md9040603

来源: mdpi

【 摘 要 】

Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated l-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.