Pulsed Ultraviolet Light Reduces Immunoglobulin E Binding to Atlantic White Shrimp (Litopenaeus setiferus) Extract

International Journal of Environmental Research and Public Health

Sandra Shriver¹  Wade Yang¹  Si-Yin Chung² 

[1] Department of Food Science & Human Nutrition, University of Florida, P.O. Box 110370, 359 FSHN Bldg. Newell Drive, Gainesville, FL 32611, USA; E-Mails:;Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd., Bldg 001 SRRC, New Orleans, LA 70124, USA; E-Mail:

关键词: allergen;    allergy;    shrimp;    pulsed ultraviolet light;    PUV;    tropomyosin;    IgE antibodies;   

DOI  :  10.3390/ijerph8072569

来源: mdpi

【 摘 要 】

Pulsed ultraviolet light (PUV), a novel food processing and preservation technology, has been shown to reduce allergen levels in peanut and soybean samples. In this study, the efficacy of using PUV to reduce the reactivity of the major shrimp allergen, tropomyosin (36-kDa), and to attenuate immunoglobulin E (IgE) binding to shrimp extract was examined. Atlantic white shrimp (Litopenaeus setiferus) extract was treated with PUV (3 pulses/s, 10 cm from light source) for 4 min. Tropomyosin was compared in the untreated, boiled, PUV-treated and [boiled+PUV]-treated samples, and changes in the tropomyosin levels were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE binding of the treated extract was analyzed via immunoblot and enzyme-linked immunosorbent assay (ELISA) using pooled human plasma containing IgE antibodies against shrimp allergens. Results showed that levels of tropomyosin and IgE binding were reduced following PUV treatment. However, boiling increased IgE binding, while PUV treatment could offset the increased allergen reactivity caused by boiling. In conclusion, PUV treatment reduced the reactivity of the major shrimp allergen, tropomyosin, and decreased the IgE binding capacity of the shrimp extract.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland