Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies

Toxins

Baozhu Guo⁴  Natalie D. Fedorova³  Xiaoping Chen¹  Chun-Hua Wan³  Wei Wang³  William C. Nierman³  Deepak Bhatnagar² 

[1]Department of Plant Pathology, The University of Georgia, Tifton, GA 31793-0748, USA

[2]Southern Regional Research Center, U.S. Department of Agriculture-Agricultural Research Service, New Orleans, LA 70124, USA

[3]The J Craig Venter Institute, Rockville, MD 20850-3343, USA

[4]Crop Protection and Management Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Tifton, GA 31794, USA

关键词: EST;    microarray;    gene profiling;    peanut-fungus interaction;    resistance genes;    Aspergillus flavus;    A. parasiticus;    metarep;   

DOI  :  10.3390/toxins3070737

来源: mdpi

【 摘 要 】

Aspergillus flavus and A. parasiticus infect peanut seeds and produce aflatoxins, which are associated with various diseases in domestic animals and humans throughout the world. The most cost-effective strategy to minimize aflatoxin contamination involves the development of peanut cultivars that are resistant to fungal infection and/or aflatoxin production. To identify peanut Aspergillus-interactive and peanut Aspergillus-resistance genes, we carried out a large scale peanut Expressed Sequence Tag (EST) project which we used to construct a peanut glass slide oligonucleotide microarray. The fabricated microarray represents over 40% of the protein coding genes in the peanut genome. For expression profiling, resistant and susceptible peanut cultivars were infected with a mixture of Aspergillus flavus and parasiticus spores. The subsequent microarray analysis identified 62 genes in resistant cultivars that were up-expressed in response to Aspergillus infection. In addition, we identified 22 putative Aspergillus-resistance genes that were constitutively up-expressed in the resistant cultivar in comparison to the susceptible cultivar. Some of these genes were homologous to peanut, corn, and soybean genes that were previously shown to confer resistance to fungal infection. This study is a first step towards a comprehensive genome-scale platform for developing Aspergillus-resistant peanut cultivars through targeted marker-assisted breeding and genetic engineering.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.