A Public Platform for the Verification of the Phenotypic Effect of Candidate Genes for Resistance to Aflatoxin Accumulation and Aspergillus flavus Infection in Maize

Toxins

Marilyn L. Warburton¹  William Paul Williams¹  Leigh Hawkins¹  Susan Bridges²  Cathy Gresham²  Jonathan Harper²  Seval Ozkan³  J. Erik Mylroie³ 

[1] Corn Host Plant Resistance Research Unit, U.S. Department of Agriculture-Agricultural Research Service, MS 39762, USA;Department of Computer Science and Engineering, Mississippi State University, MS 39762, USA;Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, MS 39762, USA;

关键词: aflatoxin;    Aspergillus flavus;    host plant resistance;    candidate gene validation;   

DOI  :  10.3390/toxins3070754

来源: mdpi

【 摘 要 】

A public candidate gene testing pipeline for resistance to aflatoxin accumulation or Aspergillus flavus infection in maize is presented here. The pipeline consists of steps for identifying, testing, and verifying the association of selected maize gene sequences with resistance under field conditions. Resources include a database of genetic and protein sequences associated with the reduction in aflatoxin contamination from previous studies; eight diverse inbred maize lines for polymorphism identification within any maize gene sequence; four Quantitative Trait Loci (QTL) mapping populations and one association mapping panel, all phenotyped for aflatoxin accumulation resistance and associated phenotypes; and capacity for Insertion/Deletion (InDel) and SNP genotyping in the population(s) for mapping. To date, ten genes have been identified as possible candidate genes and put through the candidate gene testing pipeline, and results are presented here to demonstrate the utility of the pipeline.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.