HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration

Molecules

Kadumi Inoue¹  Kei-ichi Kitahara¹  Yoshihiro Aikawa¹  Sadao Arai¹ 

[1] ¹Department of Human Environmental Sciences, Ochanomizu University, 2-1-1 Otuka, Bunkyo-ku, Tokyo 112-8610, Japan ²Department of Chemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan

关键词: carbohydrate;    aldose;    aldopentose;    aldohexose;    high-performance anion-exchange chromatography;   

DOI  :  10.3390/molecules16075905

来源: mdpi

【 摘 要 】

To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D₆ stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N’,N’-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pK_a values, but also the chemical structures of the cyclic aldoses.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.