Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation

Sensors

Joshua A. Hagen²  Sang N. Kim¹  Burhan Bayraktaroglu³  Kevin Leedy³  Jorge L. Chávez²  Nancy Kelley-Loughnane²  Rajesh R. Naik¹ 

[1] Materials and Manufacturing Directorate, Air Force Research Labs, Wright Patterson Air Force Base, OH 45433, USA; E-Mails:;711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Labs, Wright Patterson Air Force Base, OH 45433, USA; E-Mails:;Sensors Directorate, Air Force Research Labs, Wright Patterson Air Force Base, OH 45433, USA; E-Mails:

关键词: aptamer;    biomolecular detection;    ZnO-FET;    sensor;    riboflavin;    selectivity;    label-free;    biofunctionalization;   

DOI  :  10.3390/s110706645

来源: mdpi

【 摘 要 】

Zinc oxide field effect transistors (ZnO-FET), covalently functionalized with single stranded DNA aptamers, provide a highly selective platform for label-free small molecule sensing. The nanostructured surface morphology of ZnO provides high sensitivity and room temperature deposition allows for a wide array of substrate types. Herein we demonstrate the selective detection of riboflavin down to the pM level in aqueous solution using the negative electrical current response of the ZnO-FET by covalently attaching a riboflavin binding aptamer to the surface. The response of the biofunctionalized ZnO-FET was tuned by attaching a redox tag (ferrocene) to the 3′ terminus of the aptamer, resulting in positive current modulation upon exposure to riboflavin down to pM levels.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.