Delineating Chromosomal Breakpoints in Radiation-Induced Papillary Thyroid Cancer

Genes

Heinz-Ulrich G. Weier¹  Yuko Ito¹  Johnson Kwan¹  Jan Smida²  Jingly F. Weier¹  Ludwig Hieber³  Chun-Mei Lu¹  Lars Lehmann¹  Mei Wang⁴  Haig J. Kassabian¹  Hui Zeng¹ 

[1]Life Sciences Division, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA

[2] E-Mails:

[3]Clinical Cooperation Group Osteosarcoma, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany

[4] E-Mail:

[5]Department of Radiation Cytogenetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstr.1, Neuherberg 85764, Germany

[6] E-Mail:

[7]Department of Diabetes, City of Hope, 1500 Duarte Road, Duarte, CA 91010-3012, USA

[8] E-mail:

关键词: Chernobyl;    neoplastic disease;    papillary thyroid cancer;    translocation;    molecular cytogenetics;    breakpoint delineation;    fluorescence in situ hybridization;    bacterial artificial chromosomes;   

DOI  :  10.3390/genes2030397

来源: mdpi

【 摘 要 】

Recurrent translocations are well known hallmarks of many human solid tumors and hematological disorders, where patient- and breakpoint-specific information may facilitate prognostication and individualized therapy. In thyroid carcinomas, the proto-oncogenes RET and NTRK1 are often found to be activated through chromosomal rearrangements. However, many sporadic tumors and papillary thyroid carcinomas (PTCs) arising in patients with a history of exposure to elevated levels of ionizing irradiation do not carry these known abnormalities. We developed a rapid scheme to screen tumor cell metaphase spreads and identify candidate genes of tumorigenesis and neoplastic progression for subsequent functional studies. Using a series of overnight fluorescence in situ hybridization (FISH) experiments with pools comprised of bacterial artificial chromosome (BAC) clones, it now becomes possible to rapidly refine breakpoint maps and, within one week, progress from the low resolution Spectral Karyotyping (SKY) maps or Giemsa-banding (G-banding) karyotypes to fully integrated, high resolution physical maps including a list of candiate genes in the critical regions.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.