Induction of Apoptosis in Human Promyelocytic Leukemia HL60 Cells by Panaxynol and Panaxydol

Molecules

Zhonghong Yan¹  Ruolin Yang¹  Yi Jiang¹  Zhihui Yang¹  Junrui Yang¹  Qian Zhao¹ 

[1] ¹Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China ²Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China ³Center of Neuroscience Excellence, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

关键词: panaxynol;    panaxydol;    apoptosis;    promyelocytic leukemia cell;   

DOI  :  10.3390/molecules16075561

来源: mdpi

【 摘 要 】

Panaxynol and panaxydol are naturally occurring polyacetylenes, isolated from the lipophilic fractions of Panax notoginseng, that exert anti-proliferative effects against malignant cells. However, to the best of our knowledge, no study concerning the inhibitory effects of the two polyacetylenes on cell growth of human promyelocytic leukemia cells has been reported. In this paper, we examined the antiproliferation and proapoptotic effects of panaxynol and panaxydol on HL60 cells and investigated their mechanism of action. Cell growth inhibition of panaxynol and panaxydol were determined by trypan blue dye exclusion assays. Apoptosis of cells was revealed by morphological observation, analysis for nuclear DNA distribution and by annexin V-FITC/ PI staining using flow cytometry. It was found that panaxynol and panaxydol markedly inhibited proliferation of HL60 cells in a time- and dose-dependent manner via an apoptotic pathway. In concern with these findings, Western blot analysis showed proteolytic activation of PKCδ, caspase-3 activation and cleavage of poly (ADP [adenosine diphosphate]-ribose) polymerase in HL60 cells treated by panaxynol and panaxydol. In conclusion, panaxynol and panaxydol have profound effects on growth and apoptosis of HL60 cells, suggesting those substances are worthy of further exploration as potential anti-cancer agents.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.