Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis

International Journal of Molecular Sciences

Ce Qi¹  Yan Chen²  Qing-Zhe Jing¹ 

[1] School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; E-Mails:;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; E-Mail:

关键词: catalase;    solid lipid nanoparticles;    proteolysis;    enzyme delivery;   

DOI  :  10.3390/ijms12074282

来源: mdpi

【 摘 要 】

Catalase-loaded solid lipid nanoparticles (SLNs) were prepared by the double emulsion method (w/o/w) and solvent evaporation techniques, using acetone/methylene chloride (1:1) as an organic solvent, lecithin and triglyceride as oil phase and Poloxmer 188 as a surfactant. The optimized SLN was prepared by lecithin: triglyceride ratio (5%), 20-second + 30-second sonication, and 2% Poloxmer 188. The mean particle size of SLN was 296.0 ± 7.0 nm, polydispersity index range and zeta potential were 0.322–0.354 and −36.4 ± 0.6, respectively, and the encapsulation efficiency reached its maximum of 77.9 ± 1.56. Catalase distributed between the solid lipid and inner aqueous phase and gradually released from Poloxmer coated SLNs up to 20% within 20 h. Catalase-loaded SLN remained at 30% of H₂O₂-degrading activity after being incubated with Proteinase K for 24 h, while free catalase lost activity within 1 h.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.