Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON)

Toxins

Fang Ji¹  Hua Li¹  Jianhong Xu¹ 

[1] id="af1-toxins-03-00968">Institute of Food Safety, Jiangsu Academy of Agriculture Science (Key Lab of Agro-Food Safety and Quality, Ministry of Agriculture; Key Lab of Food Quality and Safety of Jiangsu Province—State Key Laboratory Breeding Base), Nanjing 210014, Chi

关键词: Deoxynivalenol (DON);    immunoassay;    ELISA;    Fusarium graminearum;   

DOI  :  10.3390/toxins3080968

来源: mdpi

【 摘 要 】

Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high risk of DON ingestion and the possibility of frequent exposure, it is important to develop a rapid and highly sensitive method for easy identification and quantification of DON in grain samples. In this study, we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) to detect DON in wheat. We conjugated 3-O-Hemisuccinyl-DON (3HS-DON) to Bovine serum albumin (BSA) and Ovalbumin (OVA), and obtained DON-specific mice antisera. The indirect competitive ELISA revealed that the optimal concentration of mice serum and the coated antigen was 1/1600 and 1/1500, respectively. The antiserum cross-reacted with the trichothecenes 3-acetyl-DON and T-2 toxin, reaching about 55.2% and 6.3%, respectively, as compared with DON. Results showed that the assay could be performed satisfactorily using an extraction buffer containing less than 15% methanol. Recovery from DON was 82–93% in grains. The linear detection range of DON in grains was between 0.01 and 100 μg/mL.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.