Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface

Sensors

Gang Liu³  Ruojun Lao¹  Li Xu³  Qin Xu³  Lanying Li³  Min Zhang³  Hao Shen²  Sanjay Mathur²  Chunhai Fan¹ 

[1] Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; E-Mails:;Institute of Inorganic Chemistry, University of Cologne, Cologne 50939, Germany; E-Mails:;Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China; E-Mails:

关键词: electrochemical biosensor;    single-nucleotide polymorphism (SNP);    nonfouling electrode surface;    Escherichia coli;   

DOI  :  10.3390/s110808018

来源: mdpi

【 摘 要 】

We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.