Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds

Materials

Ljudmilla Borisjuk²  Hardy Rolletschek²  Johannes Fuchs²  Gerd Melkus¹ 

[1] Department of Experimental Physics 5 (Biophysics), University of Würzburg, Am Hubland, Würzburg D-97074, Germany; E-Mail:;Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben 06466, Germany; E-Mails:

关键词: NMR;    MRI;    seed quality;    crop seed;    lipid imaging;    sucrose allocation;    seed aging;    ¹³C;   

DOI  :  10.3390/ma4081426

来源: mdpi

【 摘 要 】

Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.