β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells

Cancers

Nibedita Gupta¹  Fee Schmitt¹  Sina Grebhardt¹ 

[1] Hormones and Signal Transduction Group, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany;

关键词: β-catenin;    estrogen receptor;    cross-talk;    gene expression;    transcriptional activity;    breast cancer cells;   

DOI  :  10.3390/cancers3032990

来源: mdpi

【 摘 要 】

Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.