Identification and Role of Regulatory Non-Coding RNAs in Listeria monocytogenes

International Journal of Molecular Sciences

Benjamin Izar¹  Mobarak Abu Mraheil¹ 

[1]Institute of Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, Giessen 35392, Germany

[2] E-Mails:

关键词: Listeria monocytogenes;    sRNA;    asRNA;    riboswitch;    non-coding RNA;    regulatory RNA;    RNA-Seq;    whole genome tiling arrays;    infection;    UTR;   

DOI  :  10.3390/ijms12085070

来源: mdpi

【 摘 要 】

Bacterial regulatory non-coding RNAs control numerous mRNA targets that direct a plethora of biological processes, such as the adaption to environmental changes, growth and virulence. Recently developed high-throughput techniques, such as genomic tiling arrays and RNA-Seq have allowed investigating prokaryotic cis- and trans-acting regulatory RNAs, including sRNAs, asRNAs, untranslated regions (UTR) and riboswitches. As a result, we obtained a more comprehensive view on the complexity and plasticity of the prokaryotic genome biology. Listeria monocytogenes was utilized as a model system for intracellular pathogenic bacteria in several studies, which revealed the presence of about 180 regulatory RNAs in the listerial genome. A regulatory role of non-coding RNAs in survival, virulence and adaptation mechanisms of L. monocytogenes was confirmed in subsequent experiments, thus, providing insight into a multifaceted modulatory function of RNA/mRNA interference. In this review, we discuss the identification of regulatory RNAs by high-throughput techniques and in their functional role in L. monocytogenes.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.