Molecules | |
Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid | |
Maria Elisa Melo Branco de Araújo1  Fabiano Jares Contesini1  Yollanda Edwirges Moreira Franco1  Alexandra C.H. Frankland Sawaya1  Thiago Grando Alberto1  Natália Dalfré1  | |
[1] 1São Francisco University, São Francisco de Assis Av., 218, Bragança Paulista, SP 12916-900, Brazil | |
关键词: Candida antarctica; lipase; hesperidin; acylation; | |
DOI : 10.3390/molecules16087171 | |
来源: mdpi | |
【 摘 要 】
Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.
【 授权许可】
CC BY
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
【 预 览 】
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RO202003190048213ZK.pdf | 545KB | download |