Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

Molecules

Maria Elisa Melo Branco de Araújo¹  Fabiano Jares Contesini¹  Yollanda Edwirges Moreira Franco¹  Alexandra C.H. Frankland Sawaya¹  Thiago Grando Alberto¹  Natália Dalfré¹ 

[1] ¹São Francisco University, São Francisco de Assis Av., 218, Bragança Paulista, SP 12916-900, Brazil

关键词: Candida antarctica;    lipase;    hesperidin;    acylation;   

DOI  :  10.3390/molecules16087171

来源: mdpi

【 摘 要 】

Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF₄. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF₄/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF₄ and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.