Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis

Marine Drugs

Baldo F. Cordero¹  Irina Obraztsova¹  Inmaculada Couso¹  Rosa Leon²  Maria Angeles Vargas¹ 

[1] Institute of Plant Biochemistry and Photosynthesis, CIC Cartuja, University of Seville and CSIC, Avda. Americo Vespucio no. 49, 41092-Seville, Spain; E-Mails:;Department of Chemistry, Experimental Sciences Faculty, University of Huelva, Avda. Fuerzas Armadas s/n, 21071-Huelva, Spain; E-Mail:

关键词: microalgae;    carotenoids;    batch culture;    mixotrophic culture;    N-methyl-N′-nitro-nitrosoguanidine;    high lutein yielding mutants;   

DOI  :  10.3390/md9091607

来源: mdpi

【 摘 要 】

Chlorella sorokiniana has been selected for lutein production, after a screening of thirteen species of microalgae, since it showed both a high content in this carotenoid and a high growth rate. The effects of several nutritional and environmental factors on cell growth and lutein accumulation have been studied. Maximal specific growth rate and lutein content were attained at 690 μmol photons m⁻² s⁻¹, 28 °C, 2 mM NaCl, 40 mM nitrate and under mixotrophic conditions. In general, optimal conditions for the growth of this strain also lead to maximal lutein productivity. High lutein yielding mutants of C. sorokiniana have been obtained by random mutagenesis, using N-methyl-N′-nitro-nitrosoguanidine (MNNG) as a mutagen and selecting mutants by their resistance to the inhibitors of the carotenogenic pathway nicotine and norflurazon. Among the mutants resistant to the herbicides, those exhibiting both high content in lutein and high growth rate were chosen. Several mutants exhibited higher contents in this carotenoid than the wild type, showing, in addition, either a similar or higher growth rate than the latter strain. The mutant MR-16 exhibited a 2.0-fold higher volumetric lutein content than that of the wild type, attaining values of 42.0 mg L⁻¹ and mutants DMR-5 and DMR-8 attained a lutein cellular content of 7.0 mg g⁻¹ dry weight. The high lutein yield exhibited by C. sorokiniana makes this microalga an excellent candidate for the production of this commercially interesting pigment.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland