Helichrysum gymnocephalum Essential Oil: Chemical Composition and Cytotoxic, Antimalarial and Antioxidant Activities, Attribution of the Activity Origin by Correlations

Molecules

Samia Afoulous¹  Hicham Ferhout¹  Emmanuel Guy Raoelison¹  Alexis Valentin¹  Béatrice Moukarzel¹  François Couderc¹ 

[1] ¹Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique UMR CNRS 5623, University of Toulouse, University of Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, France

关键词: Helichrysum gymnocephalum;    GC-MS;    antimalarial activity;    cytotoxic activity;    antioxidant activity;   

DOI  :  10.3390/molecules16108273

来源: mdpi

【 摘 要 】

Helichrysum gymnocephalum essential oil (EO) was prepared by hydrodistillation of its leaves and characterized by GC-MS and quantified by GC-FID. Twenty three compounds were identified. 1,8-Cineole (47.4%), bicyclosesquiphellandrene (5.6%), γ-curcumene (5.6%), α-amorphene (5.1%) and bicyclogermacrene (5%) were the main components. Our results confirmed the important chemical variability of H. gymnocephalum. The essential oil was tested in vitro for cytotoxic (on human breast cancer cells MCF-7), antimalarial (Plasmodium falciparum: FcB1-Columbia strain, chloroquine-resistant) and antioxidant (ABTS and DPPH assays) activities. H. gymnocephalum EO was found to be active against MCF-7 cells, with an IC₅₀ of 16 ± 2 mg/L. The essential oil was active against P. falciparum (IC₅₀ = 25 ± 1 mg/L). However, the essential oil exhibited a poor antioxidant activity in the DPPH (IC₅₀ value > 1,000 mg/L) and ABTS (IC₅₀ value = 1,487.67 ± 47.70 mg/L) assays. We have reviewed the existing results on the anticancer activity of essential oils on MCF-7 cell line and on their antiplasmodial activity against the P. falciparum. The aim was to establish correlations between the identified compounds and their biological activities (antiplasmodial and anticancer). β-Selinene (R² = 0.76), α-terpinolene (R² = 0.88) and aromadendrene (R² = 0.90) presented a higher relationship with the anti-cancer activity. However, only calamenene (R² = 0.70) showed a significant correlation for the antiplasmodial activity.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.