期刊论文详细信息
International Journal of Molecular Sciences
Molecular Identification of Fusarium Species in Gibberella fujikuroi Species Complex from Rice, Sugarcane and Maize from Peninsular Malaysia
Heng Mei Hsuan1  Baharuddin Salleh1 
[1] School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia; E-Mails:
关键词: Fusarium;    rice;    sugarcane;    maize;   
DOI  :  10.3390/ijms12106722
来源: mdpi
PDF
【 摘 要 】

The objective of this study was to identify Fusarium species in the Gibberella fujikuroi species complex from rice, sugarcane and maize as most of the Fusarium species in the species complex are found on the three crops. Isolates used were collected from the field and obtained from culture collection. The Fusarium isolates were initially sorted based on morphology and identifications confirmed based on the DNA sequence of the translation elongation factor 1-α (TEF-1α) gene. Based on the closest match of BLAST analysis, five species were recovered, namely, F. sacchari, F. fujikuroi, F. proliferatum, F. andiyazi and F. verticillioides. This is the first report regarding F. andiyazi from rice in Malaysia and Southeast Asia. The phylogenetic tree generated by using the neighbor joining method showed that isolates from the same species were grouped in the same clade. The present study indicated that Fusarium species in the G. fujikuroi species complex are widespread in rice, sugarcane and maize in Peninsular Malaysia. The findings also suggest that the use of morphological characters for identification of Fusarium species in the G. fujikuroi species complex from the three crops will lead to incorrect species designation.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.

【 预 览 】
附件列表
Files Size Format View
RO202003190047790ZK.pdf 291KB PDF download
  文献评价指标  
  下载次数:7次 浏览次数:17次