Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals

Sensors

Travis L. Jennings¹  Robert C. Triulzi²  Guoliang Tao²  Zachary E. St. Louis² 

[1] eBioscience, Inc., 10255 Science Center Drive, San Diego, CA 92121, USA;

关键词: quantum dot;    nanocrystal;    IHC;    immunohistochemistry;    spectral imaging;    multiplexing;    conjugation;    antibody;   

DOI  :  10.3390/s111110557

来源: mdpi

【 摘 要 】

Advances in spectral deconvolution technologies are rapidly enabling researchers to replace or enhance traditional epifluorescence microscopes with instruments capable of detecting numerous markers simultaneously in a multiplexed fashion. While significantly expediting sample throughput and elucidating sample information, this technology is limited by the spectral width of common fluorescence reporters. Semiconductor nanocrystals (NC’s) are very bright, narrow band fluorescence emitters with great potential for multiplexed fluorescence detection, however the availability of NC’s with facile attachment chemistries to targeting molecules has been a severe limitation to the advancement of NC technology in applications such as immunocytochemistry and immunohistochemistry. Here we report the development of simple, yet novel attachment chemistries for antibodies onto NC’s and demonstrate how spectral deconvolution technology enables the multiplexed detection of 5 distinct NC-antibody conjugates with fluorescence emission wavelengths separated by as little as 20 nm.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.