Supercritical Carbon Dioxide Extraction of Bioactive Compounds from Ampelopsis grossedentata Stems: Process Optimization and Antioxidant Activity

International Journal of Molecular Sciences

Yuefei Wang²  Le Ying²  Da Sun²  Shikang Zhang¹  Yuejin Zhu¹ 

[1] Hangzhou Tea Research Institute, China COOP, Hangzhou 310016, China; E-Mails:;Department of Tea Science, Zhejiang University, Hangzhou 310058, China; E-Mails:

关键词: Ampelopsis grossedentata;    stems;    bioactive compounds;    optimization;    antioxidant activity;   

DOI  :  10.3390/ijms12106856

来源: mdpi

【 摘 要 】

Supercritical carbon dioxide (SC-CO₂) extraction of bioactive compounds including flavonoids and phenolics from Ampelopsis grossedentata stems was carried out. Extraction parameters such as pressure, temperature, dynamic time and modifier, were optimized using an orthogonal array design of L₉ (3⁴), and antioxidant activities of the extracts were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and ferrous ion chelating (FIC) assay. The best conditions obtained for SC-CO₂ extraction of flavonoids was 250 bar, 40 °C, 50 min, and with a modifier of methanol/ethanol (1:3, v/v), and that for phenolics extraction was 250 bar, 40 °C, 50 min, and with a modifier of methanol/ethanol (1:1, v/v). Meantime, flavonoids and phenolics were found to be mainly responsible for the DPPH scavenging activity of the extracts, but not for the chelating activity on ferrous ion according to Pearson correlation analysis. Furthermore, several unreported flavonoids such as apigenin, vitexin, luteolin, etc., have been detected in the extracts from A. grossedentata stems.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.