N-Acetylcysteine Reduces Markers of Differentiation in 3T3-L1 Adipocytes

International Journal of Molecular Sciences

Pablo Calzadilla²  Daiana Sapochnik²  Soledad Cosentino²  Virginia Diz¹  Lelia Dicelio¹  Juan Carlos Calvo² 

[1] Departamento de Química Inorgánica y Analítica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires 1428, Argentina; E-Mails:;Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires 1428, Argentina; E-Mails:

关键词: NAC;    triglyceride;    adipocyte differentiation;   

DOI  :  10.3390/ijms12106936

来源: mdpi

【 摘 要 】

Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP β) and peroxisomal proliferator activated receptor gamma (PPAR γ) expression; we suggested that intracellular GSH content could be responsible for these effects.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.