Genetic Diversity in Fusarium graminearum from a Major Wheat-Producing Region of Argentina

Toxins

Cora Lilia Alvarez²  Stefania Somma¹  Robert H. Proctor³  Gaetano Stea¹  Giuseppina Mulè¹  Antonio F. Logrieco¹  Virginia Fernandez Pinto² 

[1] Institute of Sciences of Food Production, CNR, Via Amendola 122/0, 70126 Bari, Italy;Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Buenos Aires, Argentina;Bacterial Foodborne Pathogen and Mycology Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL 61604, USA;

关键词: Fusarium Head Blight;    AFLP;    translation elongation factor 1−α;    β-tubulin;    deoxynivalenol;    3-acetyldeoxynivalenol;    15-acetyldeoxynivalenol;   

DOI  :  10.3390/toxins3101294

来源: mdpi

【 摘 要 】

The Fusarium graminearum species complex (FGSC) is a group of mycotoxigenic fungi that are the primary cause of Fusarium head blight (FHB) of wheat worldwide. The distribution, frequency of occurrence, and genetic diversity of FGSC species in cereal crops in South America is not well understood compared to some regions of Asia, Europe and North America. Therefore, we examined the frequency and genetic diversity of a collection of 183 FGSC isolates recovered from wheat grown during multiple growing seasons and across a large area of eastern Argentina, a major wheat producing region in South America. Sequence analysis of the translation elongation factor 1−α and β-tubulin genes as well as Amplified Fragment Length Polymorphism (AFLP) analyses indicated that all isolates were the FGSC species F. graminearum sensu stricto. AFLP analysis resolved at least 11 subgroups, and all the isolates represented different AFLP haplotypes. AFLP profile and geographic origin were not correlated. Previously obtained trichothecene production profiles of the isolates revealed that the 15-acetyldeoxynivalenol chemotype was slightly more frequent than the 3-acetyldeoxynivalenol chemotype among the isolates. These data extend the current understanding of FGSC diversity and provide further evidence that F. graminearum sensu stricto is the predominant cause of FHB in the temperate main wheat-growing area of Argentina. Moreover, two isolates of F. crookwellense and four of F. pseudograminearum were also recovered from wheat samples and sequenced. The results also suggest that, although F. graminearum sensu stricto was the only FGSC species recovered in this study, the high level of genetic diversity within this species should be considered in plant breeding efforts and development of other disease management strategies aimed at reducing FHB.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland