Development of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species

International Journal of Molecular Sciences

Heng-Sheng Lin²  Chih-Yun Chiang²  Song-Bin Chang¹ 

[1] Department of Life Sciences, Institute of Biodiversity, National Cheng Kung University, Tainan 70101, Taiwan;

关键词: model system;    Setaria italica;    SSRs;    Taiwan;    transferability;   

DOI  :  10.3390/ijms12117835

来源: mdpi

【 摘 要 】

Foxtail millet is one of the world’s oldest cultivated crops. It has been adopted as a model organism for providing a deeper understanding of plant biology. In this study, 45 simple sequence repeats (SSR) markers of Setaria italica were developed. These markers showing polymorphism were screened in 223 samples from 12 foxtail millet populations around Taiwan. The most common dinucleotide and trinucleotide repeat motifs are AC/TG (84.21%) and CAT (46.15%). The average number of alleles (N_a), the average heterozygosities observed (H_o) and expected (H_e) are 3.73, 0.714, 0.587, respectively. In addition, 24 SSR markers had shown transferability to six related Poaceae species. These new markers provide tools for examining genetic relatedness among foxtail millet populations and other related species. It is suitable for germplasm management and protection in Poaceae.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.