Rapid and Green Analytical Method for the Determination of Quinoline Alkaloids from Cinchona succirubra Based on Microwave-Integrated Extraction and Leaching (MIEL) Prior to High Performance Liquid Chromatography

International Journal of Molecular Sciences

Anne-Sylvie Fabiano-Tixier¹  Abdelhakim Elomri²  Axelle Blanckaert²  Elisabeth Seguin²  Emmanuel Petitcolas¹ 

[1] UMR 408 INRA, Safety and Quality of Plant Products, University of Avignon, 84000 Avignon, France; E-Mails:;CNRS UMR 6014, C.O.B.R.A., UFR Medicine-Pharmacy, University of Rouen, 76183 Rouen, France; E-Mails:

关键词: Cinchona alkaloids;    Soxhlet;    microwave;    extraction;    HPLC;   

DOI  :  10.3390/ijms12117846

来源: mdpi

【 摘 要 】

Quinas contains several compounds, such as quinoline alkaloids, principally quinine, quinidine, cinchonine and cichonidine. Identified from barks of Cinchona, quinine is still commonly used to treat human malaria. Microwave-Integrated Extraction and Leaching (MIEL) is proposed for the extraction of quinoline alkaloids from bark of Cinchona succirubra. The process is performed in four steps, which ensures complete, rapid and accurate extraction of the samples. Optimal conditions for extraction were obtained using a response surface methodology reached from a central composite design. The MIEL extraction has been compared with a conventional technique soxhlet extraction. The extracts of quinoline alkaloids from C. succirubra obtained by these two different methods were compared by HPLC. The extracts obtained by MIEL in 32 min were quantitatively (yield) and qualitatively (quinine, quinidine, cinchonine, cinchonidine) similar to those obtained by conventional Soxhlet extraction in 3 hours. MIEL is a green technology that serves as a good alternative for the extraction of Cinchona alkaloids.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.