Microsatellite Analysis in Multistage Carcinogenesis of Esophageal Squamous Cell Carcinoma from Chongqing in Southern China

International Journal of Molecular Sciences

Ming Liu³  Feng Zhang¹  Shen Liu²  Wen Zhao⁴  Jing Zhu² 

[1] Beijing Institute of Genomics of the Chinese Academy of Sciences, Beijing 100029, China; E-Mail:;Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University, Chongqing 400038, China; E-Mails:;Cardiothoracic Surgery Department, Affiliated South-West Hospital, Third Military Medical University, Chongqing 400038, China; E-Mail:;Pathology Department, Affiliated South-West Hospital, Third Military Medical University, Chongqing 400038, China; E-Mail:

关键词: esophageal squamous cell carcinoma;    microsatellite;    loss of heterozygosity;   

DOI  :  10.3390/ijms12117401

来源: mdpi

【 摘 要 】

In order to characterize the molecular events in the carcinogenesis of esophageal cancer and to identify biomarkers for the early detection of the disease, matched precancerous and cancerous tissues resected from 34 esophageal cancer patients in Chongqing of southern China were compared for the extent of loss of heterozygosity (LOH). Sixteen microsatellite markers on nine chromosome regions were used for the PCR-based LOH analysis. The overall frequency of LOH at the 16 microsatellite loci was significantly increased as the pathological status of the resection specimens changed from low-grade dysplasia (LGD) to high-grade dysplasia (HGD) and squamous cell carcinoma (SCC) (P < 0.001), indicating that tumorigenesis of the esophageal squamous epithelia is a progressive process involving accumulative changes of LOH. A total of eight markers showed LOH in the LGD samples, suggesting that these loci may be involved in the early-stage tumorigenesis of esophageal squamous cell carcinoma (ESCC) and that LOH analysis at these loci may help improve the early detection of this disease. In addition, heterozygosity was regained at four loci in the SCC samples of four patients compared with the HGD samples, suggesting the possibility of genetic heterogeneity in the tumorigenesis of esophageal cancer.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.