Different Reactive Oxygen Species Lead to Distinct Changes of Cellular Metal Ions in the Eukaryotic Model Organism Saccharomyces cerevisiae

International Journal of Molecular Sciences

Ming J. Wu²  Patrick J. O𠆝oherty²  Patricia A. Murphy²  Victoria Lyons²  Melinda Christophersen¹  Peter J. Rogers¹  Trevor D. Bailey² 

[1] Carlton and United Breweries, Fosters Group, 4-6 Southampton Crescent, Abbotsford, Melbourne 3067, Australia; E-Mails:;School of Biomedical and Health Sciences, College of Health and Science, University of Western Sydney, Locked Bag 1797, Penrith South DC, New South Wales 1797, Australia; E-Mails:

关键词: reactive oxygen species;    metal ions;    ionomic profiling;    yeast;    Saccharomyces cerevisiae;   

DOI  :  10.3390/ijms12118119

来源: mdpi

【 摘 要 】

Elemental uptake and export of the cell are tightly regulated thereby maintaining the ionomic homeostasis. This equilibrium can be disrupted upon exposure to exogenous reactive oxygen species (ROS), leading to reduction or elevation of the intracellular metal ions. In this study, the ionomic composition in the eukaryotic model organism Saccharomyces cerevisiae was profiled using the inductively-coupled plasma optical emission spectrometer (ICP-OES) following the treatment with individual ROS, including hydrogen peroxide, cumen hydroperoxide, linoleic acid hydroperoxide (LAH), the superoxide-generating agent menadione, the thiol-oxidising agent diamide [diazine-dicarboxylic acid-bis(dimethylamide)], dimedone and peroxynitrite. The findings demonstrated that different ROS resulted in distinct changes in cellular metal ions. Aluminium (Al³⁺) level rose up to 50-fold after the diamide treatment. Cellular potassium (K⁺) in LAH-treated cells was 26-fold less compared to the non-treated controls. The diamide-induced Al³⁺ accumulation was further validated by the enhanced Al³⁺ uptake along the time course and diamide doses. Pre-incubation of yeast with individual elements including iron, copper, manganese and magnesium failed to block diamide-induced Al³⁺ uptake, suggesting Al³⁺-specific transporters could be involved in Al³⁺ uptake. Furthermore, LAH-induced potassium depletion was validated by a rescue experiment in which addition of potassium increased yeast growth in LAH-containing media by 26% compared to LAH alone. Taken together, the data, for the first time, demonstrated the linkage between ionomic profiles and individual oxidative conditions.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.