Effect of Byrsonima crassa and Phenolic Constituents on Helicobacter pylori-Induced Neutrophils Oxidative Burst

International Journal of Molecular Sciences

Cibele Bonacorsi¹  Maria Stella G. Raddi¹  Luiz Marcos da Fonseca¹  Miriam Sannomiya² 

[1] School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, 14801-902, Brazil; E-Mail:;School of Arts, Sciences and Humanities, University of São Paulo (USP), São Paulo, SP, 03828-000, Brazil; E-Mail:

关键词: Byrsonima crassa;    Helicobacter pylori;    gastric ulcers;    antioxidant activity;    phenolic compounds;    chemiluminescence;   

DOI  :  10.3390/ijms13010133

来源: mdpi

【 摘 要 】

Byrsonima crassa Niedenzu (Malpighiaceae) is used in Brazilian folk medicine for the treatment of diseases related mainly to gastric ulcers. In a previous study, our group described the gastric protective effect of the methanolic extract from the leaves of B. crassa. The present study was carried out to investigate the effects of methanolic extract and its phenolic compounds on the respiratory burst of neutrophils stimulated by H. pylori using a luminol-based chemiluminescence assay as well as their anti-H. pylori activity. The suppressive activity on oxidative burst of H. pylori-stimulated neutrophils was in the order of methyl gallate > (+)-catechin > methanol extract > quercetin 3-O-α-l-arabinopyranoside > quercetin 3-O-β-d-galactopyranoside > amentoflavone. Methyl gallate, compound that induced the highest suppressive activity with IC₅₀ value of 3.4 μg/mL, did not show anti-H. pylori activity. B. crassa could be considered as a potential source of natural antioxidant in gastric ulcers by attenuating the effects on the damage to gastric mucosa caused by neutrophil generated reactive oxygen species, even when H. pylori displays its evasion mechanisms.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.